«Environmental protection in oil and gas complex»

Environmental monitoring of oil and gas complex facilities using bacterial viability indicators

UDC: 550.47-550.378+320.193.8
DOI: -

Authors:

¹ Nizhny Novgorod State Technical University named after R.E. Alekseev, Nizhny Novgorod, Russia
² Nizhny Novgorod State University named after N.I. Lobachevsky, Nizhny Novgorod, Russia

Keywords: methylthiazolyl tetrazolium (MTT) bromide, organotrophic bacteria, respiratory activity of bacteria, environmental monitoring, oil and gas industry, tetrazolium salts, formazan, indicator of bacterial viability, kinetics, inhibition

Annotation:

The authors of the article kinetically studied the impact of a suspension in a physiological solution of bacteria, widely represented under native conditions, on methylthiazolinetetrazolium (MTT), including soil near the facilities of oil and gas complex It was revealed that the initial rate of MTT restoration by the suspension of Bacillus bacteria cells in a physiological solution does not depend on the initial concentration of the tetrazolium salt. On this basis, the viability of bacteria can be studied at low concentrations of the indicator salt. The response of viable Bacillus bacteria to MTT is manifested without induction during a short exposure time even in the absence of an external nourish source. It is of grate important when monitoring the environment state, especially in case of negative consequences of emergency situations at oil and gas enterprises. The effect of inhibition of bacterial cells respiratory activity by methylthiazolylformazan revealed by the kinetic method can also be used to solve the inverse problem – for effective suppression of bacterial viability.

Bibliography:

1. Altman F.P. Tetrazolium salts and formazans. – Stuttgart; New York: Gustav Fisher, 1976. – 56 p.
2. Seidler E. The Tetrazolium-Formazan System: Design and Histochemistry. – Stuttgart; New York: Gustav Fisher, 1991. – 79 p.
3. Kuhn R., Jerchel D. Reduktion von Tetrazoliumsalzen durch Bakterier, gärende Hefe und keimende Samen // Berichte der deutschen chemischen Gesellschaft (A and B Series). – 1941. – Vol. 74, No. 6. – S. 949–952. – DOI: 10.1002/cber.19410740615
4. Comparing different methods for fast screening of microbiological quality of beach sand aimed at rapid-response remediation / R.C. Testolin, T.C.M. de Almeida, M. Polette [et al.] // Marine Pollution Bulletin. – 2017. – Vol. 118, Issue 1-2. – P. 206–212. – DOI: 10.1016/j.marpolbul.2017.02.069
5. Kruglov Yu.V. Mikrobnoe soobshchestvo pochvy: fiziologicheskoe raznoobrazie i metody issledovaniya // Sel'skokhozyaystvennaya biologiya. – 2016. – T. 51, № 1. – S. 46–59. – DOI: 10.15389/agrobiology.2016.1.46rus
6. Development of a Bacterial-based Tetrazolium Dye (MTT) Assay for Monitoring of Heavy Metals / H.W.M. Isa, W.L.W. Johari, A. Syahir [et al.] // Int. J. of Agriculture and Biology. – 2014. – Vol. 16, Issue 6. – P. 1123–1128.
7. Prokonova T.V. Poisk aktual'nykh bioindikatorov dlya kompleksnoy otsenki ekologicheskogo sostoyaniya landshafta na territorii vozdeystviya tekhnogennogo podzemnogo khranilishcha gaza // Zashchita okruzhayushchey sredy v neftegazovom komplekse. – 2022. – № 2(305). – S. 31–35. – DOI: 10.33285/2411-7013-2022-2(305)-31-35
8. Ermakov V.V., Ivashchenko I.S. Trebovaniya k sistemam nepreryvnogo monitoringa neftezagryaznennykh territoriy // Tr. RGU nefti i gaza im. I.M. Gubkina. – 2020. – № 3(300). – S. 97–103. – DOI: 10.33285/2073-9028-2020-3(300)-97-103
9. Pat. 2335543 Ros. Federatsiya, MPK C12Q 1/02. Sposob mul'tisubstratnogo testirovaniya mikrobnykh soobshchestv i ego primenenie / M.V. Gorlenko, P.A. Kozhevin, A.S. Terekhov; patentoobladatel' OOO "Ekologicheskiy tsentr "Eko-terra". – № 2006124312/13; zayavl. 07.07.2006; opubl. 10.10.2008, Byul. № 28.
10. Gorlenko M.V., Kozhevin P.A. Mul'tisubstratnoe testirovanie prirodnykh mikrobnykh soobshchestv. – M.: MAKS Press, 2005. – 88 s.
11. Beleneva I.A., Kharchenko U.V. Primenenie metoda mul'tisubstratnogo testirovaniya sostoyaniya morskikh poverkhnostnykh vod // Biologiya morya. – 2018. – T. 44, № 3. – S. 172–178.
12. Suktsessiya mikrobnogo soobshchestva seroy lesnoy pochvy pri razlozhenii razlichnykh organicheskikh soedineniy / T.E. Khomutova, K.S. Dushchanova, V.E. Smirnov, A.V. Borisov // Pochvovedenie. – 2019. – № 8. – S. 966–973. – DOI: 10.1134/S0032180X19080082
13. Vliyanie vidovykh razlichiy bakteriy rodov Bacillus i Pseudomonas, suspendirovannykh v fiziologicheskom rastvore, na skorost' vosstanovleniya iodnitrotetrazoliya khlorida / A.A. Kalinina, N.V. Gurskiy, T.N Sokolova [i dr.] // Teoreticheskaya i prikladnaya ekologiya. – 2022. – № 1. – S. 145–152. – DOI: 10.25750/1995-4301-2022-1-145-152
14. Application of chemical kinetics methods to the study of iodonitrotetrazolium chloride reduction in the presence of Bacillus subtilis cells / A.A. Kalinina, A.S. Makedoshin, S.Y. Radostin [et al.] // Moscow University Chemistry Bulletin. – 2019. – Vol. 74, No. 1. – P. 20–24. – DOI: 10.3103/S0027131419010048
15. Kineticheskoe issledovanie vosstanovleniya iodnitrotetrazoliya khlorida suspenziey v fiziologicheskom rastvore gramotritsatel'nykh bakteriy Pseudomonas aeruginosa i Escherichia coli / A.A. Kalinina, A.S. Makedoshin, N.V. Gurskiy [i dr.] // Teoreticheskaya i prikladnaya ekologiya. – 2018. – № 1. – S. 25–32.
16. Vajrabhaya L., Korsuwannawong S. Cytotoxicity evaluation of a Thai herb using tetrazolium (MTT) and sulforhodamine B (SRB) assays // J. of Analytical Science and Technology. – 2018. – Vol. 9, No. 1. – DOI: 10.1186/s40543-018-0146-0
17. In vitro methodological standardization of MTT colorimetric assay for evaluation of formazan activity and drug dosage: Preprint / L. Nepomuceno, L.S. de Carvalho, N.P. Soares [et al.] // Research Square. – 2020. – 16 p. – DOI: 10.21203/rs.2.20775/v1
18. Use of the tetrazolium salt MTT to measure cell viability effects of the bacterial antagonist Lysobacter enzymogenes on the filamentous fungus Cryphonectria parasitica / N. Patel, P.V. Oudemans, B.I. Hillman, D.Y. Kobayashi // Antonie van Leeuwenhoek. – 2013. – Vol. 103, Issue 6. – P. 1271–1280. – DOI: 10.1007/s10482-013-9907-3
19. Benov L. Effect of growth media on the MTT colorimetric assay in bacteria // PLoS One. – 2019. – Vol. 14, No. 8. – P. e0219713. – DOI: 10.1371/journal.pone.0219713